In sporophytically self - incompatible brassica , three s - linked genes , the pollen ligand scr , stigma receptor slg and srk , have been identified . in addition , four genes ( arc1 , thl1 , thl2 and mod ) that do not link to s locus have also been found , they are regarded as downstream protein factors of self - incompatibility ( si ) response , however , their biological function is not clear 在孢子体型自交不亲和的芸薹属中，已经鉴定出3个主要与s位点紧密连锁的基因，花粉中的配体scr和柱头中的受体slg与srk ：另外鉴定出4个不与s位点连锁的基因arc1 、 thl1 、 thl2和mod ，它们被认为是si反应的下游蛋白质因子，但是对它们的生物学功能了解的还比较少。
Arc1 , thl1 and thl2 , the substrate protein genes of s receptor kinase , were cloned through a series of methods of molecular biology such as pcr , rt - pcr , dna cloning and sequencing . the resultings sequences were highly analysed by using the related biosoftwares on internet , providing new insights in the field of the molecular mechanism of self - incompatibility in plants . the major results are as followings : 1 本文通过pcr和rt - pcr等一系列分子生物学方法克隆了芸薹属植物中的甘蓝和油菜自交不亲和信号传导过程中srk底物蛋白基因arc1 、 thl1和thl2 ，并使用各种相关生物信息学软件对srk底物蛋白基因序列进行了分析，然后在internet网上利用在线软件对蛋白质的结构和功能进行了预测和探讨，以期为芸薹属植物自交不亲和性的分子机理的研究提供新的内容。
The method of ctab was specifically improved for the extraction of genomic dna and total rnas from brassica oleracea l . and brassica napus l . 2 . arclboler gene was amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) 以甘蓝( 200110197 )和油菜( y578 - 2 )自交不亲和系的植株为材料，提取幼苗中的gdna及花蕾期的柱头、子房、花瓣、叶片、根的mrna ，并对mrna进行反转录合成cdna ，然后分别以gdna与cdna为模板扩增arc1蛋白编码序列。
The self - incompatibility is involved in a signal transduction process resulted from the interaction between ligand and receptor . up to now , some achievements for ligand and receptor have already been made , but the research progress on downstream protein factors is slower than that of upstream components , highlighting the downstream proteins in priority of research . some downstream components have not been isolated , which are similar to the unknown function of those isolated ones 自交不亲和反应是一种配体和受体相互识别的信号传导过程，在这一过程中，对配体和受体的研究已经取得突破性成果，而对下游的磷酸化蛋白研究相对上游受体来说要缓慢得多，所以对信号传导的下游组分研究显得尤为重要，特?是下游还有许多未分离到的组分，而分离到的组分其功能还不清楚。
These bacteria are transmitted through the cytoplasm of eggs and have evolved various mechanisms for manipulating reproduction of their hosts , including cytoplasimic incompatibility ( ci ) , parthenogenesis , feminization , male killing , fecundity or fertility modifying etc . wolbachia are common and widespread bacteria infecting 17 % insect population , 17 crustacean species , mites and many nematodes 这些共生菌通过母系遗传参与调控寄主的多种生殖活动，包括细胞质不亲和现象、诱导孤雌生殖现象、雌性化、雄性致死以及调节寄主生殖力等。 wolbachia的分布十分广泛，目前已经发现在超过17的昆虫种群以及17种甲壳纲动物、螨虫、线虫体内发现了它的存在。
" xiyuan4 " , a b . olereacea l . and " 2001817 " , a b . napus l . were bred by southwest agricultural university . here using them as materials , we studied and explored thoroughly the dna and cdna sequences of thl2 gene and mod gene with bioinformatical methods . for the first time , based on sequence data , we analyzed the structure , heredity and variation of the dna sequences encoding thl2 and mod , and foresaw the molecular mechanism in self - incompatibility signal process 本研究以西南农业大学繁育的高度自交不亲和的‘西园4号’甘蓝和‘ 2001817 ’油菜为试材，采用生物信息学的方法全面研究和探索性的分析了thl2基因和mod基因的dna与cdna序列，首次从序列数据出发研究这两个编码基因的结构与遗传变异，以及在自交不亲和性信号转导中的作用机理
