Recently an abscisic acid binding protein with high specificity and affinity for aba was purified from epidermis of vicia faba l . in our laboratory , and further characterized using various biochemical techniques , there is evidence to link the protein to the physiological effects of aba in some aspects . the above - mentioned work may result in the molecular cloning the gene encoding aba binding protein 近年来，我室利用亲和层析从蚕豆下表皮中分离出一种高亲合、高度特异性的aba结合蛋白，并完成了对其生化特性深入分析及部分测序，且在一定程度上揭示了该蛋白与aba的生理功能效应存在着内在的联系，这为进一步克隆aba结合蛋白基因奠定了坚实的工作基础。
We use a specific no probe diaminofluorescein diacetate ( daf - 2da ) to visualize the changes of no content with different treatments and its localization . the no burst in arabidopsis was induced by vd - toxin , sa and h2o2 . no accumulated in the guard cells and the epidermal cells of the lower epidermal layer of arabidopsis 用no特异荧光染料daf - 2da染色得知，毒素、 sa 、 h _ 2o _ 2单独及组合处理拟南芥下表皮条均能诱导no的积累， no主要积累于在保卫细胞腹壁上，在保卫细胞的胞质和表皮细胞的胞壁及胞质中也有no的积累。
Both upper and lower epidermis cells are polygonal or irregular in shape in all species studied . the patterns of anticlinal walls are straight , arched or sinuous , and the raphal cuticular striastriae are occasionally observed on periclinal walls . stomatal apparatus and secretory cells are found on the upper epidermis of several species and on the lower epidermis of all species 结果表明：这些植物叶片的上、下表皮细胞呈多边形或不规则形，垂周壁式样为平直、弓形或波浪状，平周壁偶有脊状条纹；少数种类上表皮还有气孔器或分泌细胞，所有种类下表皮具气孔器和分泌细胞；气孔器类型以平列型和侧列型居多，偶尔有无规则型；气孔极区呈稍角质加厚或棒状加厚，稀t形加厚。
Changes in h2o2 generation in guard cells of vicia faba induced by aba were measured by using fluorescence probe , 8 - hydroxypyrene - l , 3 , 6 - trisulfonic acid ( hpts ) . examination of epidermis peel was performed using a laser scanning confocal microscope ( lscm ) and spectrofluorometer , set to an excitation light of 405 nm and an emission light of 512 ran 以蚕豆叶片下表皮为材料，将荧光探针hpts导入蚕豆气孔保卫细胞内，利用荧光光谱和激光共聚焦显微镜技术，检测了aba诱导蚕豆气孔关闭过程中h2o2的产生。
