The efficacy of rhgm - csf treatment on radiotherapy - induced esophagitis 巨噬细胞集落刺激因子治疗放射性食管炎的疗效
Pbmc were cultured in complete medium and supplemented with 1000u / ml rhgm - csf and 500u / ml rhil - 4 , modc were collected on day 9 . tumor cells were cultued with 10 % complete medium 肿瘤细胞的培养将mgc803 、 ls174t 、 ec109细胞分别培养于含10胎牛血清青霉素100n分ml ,链霉素100n分ml的rpmi
Surface markers on dcs were then analyzed by flow cytometry and the proliferation of t cells was detected by mtt colorimetry . resoults : peripheral blood monocytes from patients of carcinoma treated with rhgm - csf of 1000 u / ml plus il - 4 of 500 u / ml for 7 days could observe dcs with typical morphology . simultaneously there was a decrease in cd 14 expression and increase in hla - dr , cd40 , cd83 and cd86 on dcs 结果,癌症患者外周血中的单核细胞在rhgm - csf1000u ml + il - 4500u ml的条件下培养一周,就可看到典型的树突状细胞形态,其表面cd14分子表达减少, hla - dr 、 cd54 、 cd40 、 cd83及cd86分子的表达明显增高,且具有明显刺激t细胞增殖的能力,成功地完成了外周血单核细胞来源的dc的培养。
Conclusion : dendritic cells ( dcs ) can be derived from peripheral blood monocytes in vitro successfully treated with rhgm - csf of 1000 u / ml plus il - 4 of 500 u / ml . second , dcs from the patients of advanced carcinoma of large intestine were transfected with rv - cea , and then cocultured with autologous t cells . the cytotoxic activity against cea - secreting tumor cells were assessed and compared with those of t cells induced by dcs without rv - cea transfection 其次,用rv - cea转染晚期大肠癌患者的dc ,再激发自体t细胞,然后检测t细胞对cea分泌性肿瘤细胞的体外杀伤活性,并与未经rv - cea转染的dc激发的t细胞及野生型痘苗病毒转染的dc激发的t细胞进行对比。