zeocin造句
例句与造句
- after dna sequencing was correct, it was transformed into p . pastoris km71h strain by electroporation . highly resistant recombinant was isolated by increasing concentrations of zeocin ? positive clone was selected by pcr . the positive clone was induced with methanol
抗生素zeocin~(tm)浓度梯度筛选高拷贝菌株，分别提取这些高拷贝菌株的基因组dna，以此为模板做pcr反应鉴定阳性克隆。 - the positive transformants with the integrates mn-sod gene and cuzn-sod gene were identified by zeocin-resistance, pcr screening and expression in p . pastoris . the recombinant mn-sod protein and cuzn-sod protein was successfully expressed in pichia pastoris based on the evidences that the obvious activity of sod existed in native-page and enzymatic activity test
pcr鉴定进一步说明，目标基因已经重组到宿主基因组染色体上；0.5甲醇诱导表达后，活性电泳出现明显活性条带，重组酵母发酵液中mn-sod的活性约是对照菌株sod活性的2.6倍；cuzn-sod酶活力约是对照菌株sod活性的1.3倍。 - the positive transformants with the integrates mn-sod gene was identified by zeocin-resistance, pcr screening and expression in p . pastoris . the recombinant mn-sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds-page, the obvious activity of sod existed in native-page and enzymatic activity test, and mn-sod activity was specific base on the inhibition with the mixture of chloroform-enthanol ( 3 : 5 / v : v ) and potassium cyanide . two secreted plasmids ppiczaa-sodm18 and ppiczaa-sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation
pcr鉴定及mut表型分析进一步说明，目标基因已经重组到宿主菌基因组染色体上；0.5甲醇诱导表达后，sds-page结果显示，表达的蛋白相对分子量约为23kd，活性电泳出现明显活性条带；酶活性测定显示，重组菌株sod活性比对照提高5倍左右；氯仿-乙醇(3:5v:v)和kcn(5mmoll)抑制反应进一步证明，所表达的sod为锰超氧化物歧化酶。 - It's difficult to find zeocin in a sentence. 用zeocin造句挺难的
