定点整合的英文
site-directed integration
例句与用法
- The development of gene targeting and animal cloning thechnology makes the site - specific integration of foreign gene become possible
随着基因打靶技术和动物克隆技术技术的发展,外源基因在高等哺乳动物基因组中的定点整合成为了可能。 - Compared with nucleic genome , the chloroplast genome is very small and easily to be manipulated genetically , and the foreign gene is site - direct integrated into the chloroplast genome through homologous recombination
与核转化相比,叶绿体基因组小,遗传操作简单,外源基因是通过同源重组机制定点整合进叶绿体基因组。 - To determine that the integration of foreign genes had occurred hi the chloroplast genome at the directed site by homologous recombiantion and to evaluate the degree of homogeneity of the c . reinhardtii transformants , pcr and pcr - southern blot analysis were performed using the primers of c . reinhardtii chloroplast homologous fragment
并根据衣藻叶绿体同源片段chll基因,设计一对引物对其叶绿体dna进行的pcr和southernblot分析。结果表明外源基因已经定点整合入衣藻叶绿体基因组的chll基因中,并且经过三轮抗生素筛选所得的衣藻叶绿体转化子中,转基因型叶绿体基因组拷贝占多数。