ulcerans造句

• In Victoria, where most Australian cases of M . ulcerans infection occur, we have continued to use the term  Bairnsdale ulcer even though the main endemic areas are now the Bellarine and Mornington Peninsulas near Melbourne.
• After tuberculosis and leprosy, Buruli ulcer is the third most common mycobacteriosis of humans . " M . ulcerans " grows optimally on routine mycobacteriologic media at 33 癈 and elaborates a necrotizing immunosuppressive cytotoxin ( mycolactone ).
• Large ulcers almost certainly caused by " M . ulcerans " were first observed by Cook in Uganda in 1897; however, the etiologic agent was not isolated and characterized until 1948 in Australia by MacCallum and associates.
• The use of IS2404-based PCR as a detection method for " M . ulcerans " showed better sensitivity and specificity, required less time, and was less costly than the 16S rRNA gene-based PCR.
• Most " Corynebacterium " are harmless but some cause serious illness in humans, especially in immunocompromised humans . " C . ulcerans " has been known to cause diphtheria and diphtheria-like infections in patients.
• It was found that as a result of a kidney graft received the previous year, the patient was immunocompromised and vulnerable to " C . ulcerans ", which was found growing from a swab of her throat.
• The first confirmation that " M . ulcerans " was present in environmental water samples was obtained in 1997, by combining the highly sensitive and specific IS2404 PCR with a method that separated sample DNA from naturally occurring inhibitors of PCR.
• Rapid diagnostic methods for " M . ulcerans " infection, as well as methods of rapid identification of the organism in clinical and environmental specimens would be a significant advance in the management of " M . ulcerans " infection.
• Rapid diagnostic methods for " M . ulcerans " infection, as well as methods of rapid identification of the organism in clinical and environmental specimens would be a significant advance in the management of " M . ulcerans " infection.
• ""'Mycobacterium ulcerans liflandii " "'has been isolated from " Xenopus tropicalis " and " Xenopus laevis " in a laboratory in the US and causes a Mycobacterium ulcerans-like disease in anurans.
• ""'Mycobacterium ulcerans liflandii " "'has been isolated from " Xenopus tropicalis " and " Xenopus laevis " in a laboratory in the US and causes a Mycobacterium ulcerans-like disease in anurans.
• Soft tissue disease due to NTM infection include post-traumatic abscesses ( caused by rapid growers ), swimming pool granuloma ( caused by " M . marinum " ) and Buruli ulcer ( caused by " M . ulcerans " or " M . shinshuense " ).
• There was a lot of material on other mycobacterial diseases, such as Buruli ulcer, ( " Mycobacterium " " ulcerans " infections ) which is relatively unknown by the medical profession in the USA, but it is a major problem in many foci in Africa and other places such as Australia.
• Captured cells are washed to remove inhibitors and then DNA is released by standard methods prior to PCR . The third approach also uses paramagnetic beads, but here the beads are linked to " M . ulcerans "-specific oligonucleotide probes, which capture IS2404 DNA that has been released from " M . ulcerans " by homogenization and alkaline lysis.
• Captured cells are washed to remove inhibitors and then DNA is released by standard methods prior to PCR . The third approach also uses paramagnetic beads, but here the beads are linked to " M . ulcerans "-specific oligonucleotide probes, which capture IS2404 DNA that has been released from " M . ulcerans " by homogenization and alkaline lysis.
• PCR methods that have been developed are based on the 16S rRNA gene, the hsp65 gene, or the insertion sequence IS2404 . evaluated two nested PCRs : the nested IS2404-based PCR and the nested 16S rRNA gene-based PCR . IS2404-based PCR was positive only with " M . ulcerans " isolates and the closely related " M . shinshuense ".
• Mycobacterium ulcerans ", IS2404 and IS2606, were detected by PCR . These isolates could be differentiated from other slowly growing pigmented mycobacteria by their inability to grow at 37 癈, production of niacin and urease, absence of nitrate reductase, negative Tween 80 hydrolysis and resistance to isoniazid ( 1 礸 ml 1 ), p-nitrobenzoic acid, thiacetazone and thiophene-2-carboxylic hydrazide.
• He studied medicine at Guy's Hospital, London, but maintained his interest in Natural History, and had his first paper published in Medical Research Council, Barker moved with his family to Uganda, to research Mycobacterium ulcerans infection ( " Buruli ulcer " ), demonstrating that it was caused, not by mosquitos, but by wounds caused by the razor-sharp reeds growing near the River Nile.