kanamycin造句

• At the same time , phacl gene was modified by pcr . three plant expression vectors containing kanamycin photransferase gene : pc3cl ( containing phacl ) , pc3cl ( containing phac2 ) , pc3c ! c2 ( containing phacl and phact ) were constructed
  同时利用套叠pcr技术对phac1基因进行了改造，经过基因拼接构建了带有卡那霉素抗性基因的植物表达载体pc3c1 (嵌合phac1 ) 、 pc3c2 (嵌合phac2 )和pc3c1c2 (嵌合phac1和phac2双基因) 。
• The high performance two - gene expression vector pc3c ! c2 was transformed to nicotiana tobacum honghuadajinyuan plants mediated by agrobacterium tumefaciens eh a105 according to the leaf disc procedure . transformed shoots were selected on solidified medium containing l00mg / l kanamycin
  将含有phac1和phac2双基因的植物高效表达载体pc3c1c2 ，用冻融法，构建了pc3c1c2根癌土壤杆菌( agrobacteriumtumefacienseha105 )的工程菌。
• Promoter - probe vector psupv4 was used to clone promoters of pseudomonas pseudoalcaligenes in escherichia coli . nine kanamycin resistant recombinants were obtained and designated as ppal - ppa9 . the ppa7 , which has the highest kanamycin resistance , was chose for further characterization
  利用启动子探针型载体psupv4直接在大肠杆菌中克隆类产碱假单胞菌( pseudomonaspseudoalcaligenes )基因启动子片段，获得具有强启动子的重组子ppa7 。
• The growing graphs of e . coli bl21 containing pbv221 and pbv221svhb according to the od600 values showed that the e . coli vgb + grew faster 4 the highly - efficient bivalent vectors which are named pgbif4abcvhb and pgbi4asvhbbt respectively , havfs been . su ? essful ] y constructed . the tobacco leave discs were transformed with agrobacterium tumefaciens carrying the plant expression vectors pgbif4abcvhb and pgbi4asvhbbt . 38 and 36 transgenic tobacco plants have been obtained respectively after kanamycin screening , pcr detection , southern blot analysiss showed that the foreign genes have been inserted into the tocacco plant genome . the expression of 16kd vhb protein in transgenic plants was proved by western blot
  4构建了双价基因高效植物表达载体pgbif4abcvhb和pgbi4asvhbbt 。通过农杆菌介导法，将这两个双价基因高效表达载体导入烟草，分别获得了38 、 36株阳性转基因植株。经pcr 、 southernblot分子生物学方法分析，证明vgbm ， bt基因、 bt - cpti基因已整合到烟草基因组中， westernblot证明vgbm成功地表达出16kd的vhb蛋白。
• One to offspring ( t1 ) was selected for further genetic analysis from two types of self - pollinated transgenic plants and screened for the presence of the recombinant gene by the pcr respectively . the result suggested that the resistance to kanamycin can be a stable heredity by genetic analysis , separation ratio of t1 was 3 : 1 and t1 kanamycin - resistant tomato plants can carry vp7 gene
  此外，从两种转基因植株中分别选取一自交株进行t _ 1代遗传分析，其kan抗性基因分离比皆为3 : 1 ， t _ 1代抗性番茄植株的pcr结果也呈阳性。
• 3 . potato stems and agrobacterium fumefaciens containing recombinant vector were co - cultured at 28cfor 48 hours and transplanted onto callus - inducing medium at 24c for 7 days . and then , the explants were transplanted onto differentiation medium and cultured at 24c for 21 days . resistant buds rooted and grew into plants in medium with kanamycin for 20 days , and 83 plants were obtained
  3将含外源基因的根癌农杆菌与马铃薯茎段共培养后在愈伤诱导培养基上培养7天，转接到分化培养基上分化出抗性芽，抗性芽在生根培养基上生根长成完整植株，共获83株。
• 6 . transformation system of mustard a serials of kanamycin concentration was added to optimum medium to test the explants resistance capacity of two kinds of mustard . the transformation procedures described were derived from numerous regeneration and trasformation designed to test factors that might affect shoot regeneration , which including length of co - cultivation . those producing the best result parameters were described as below : after the mustard explants were precultured on regeneration medium for 2 days . they were inoculated with agrobacterium for 20 minutes . inoculated explants were co - cultivated for 4 days and in shadow at first 2 days . then transferred to the same medium plus 30 mg / l kanamycin and 500mg / l garb . all of them were transferred to fresh medium every 2 weeks . the kan - resistant plants were regenerated
  芥菜外植体高频遗传转化体系的建立在最适培养基上试验了两类芥菜的三种外植体对卡那霉素的敏感性、预培养天数、浸菌时间等因素的影响，建立了芥菜高频转基因再生体系：取生长4天的芥菜子叶、下胚轴和25天的叶片在分化培养基上( ms + ba3 . 0mg / l + naa0 . 1mg / l )预培养2 - 3天后，投入农杆菌菌液中浸染20分钟，在分化培养基上暗培养2天，正常条件下培养2天后，转入抗性培养基（ ms ba3
• Transformed shoots were selected on solidified medium containing kanamycin . ten kanamycin resistant transformants were obtained by direct or induction calla . these transformants were checked by pcr , pcr - southern blot and southern blot , confirmed that two positive transformants were integrated into the genome of boechmeria nivea l guad
  对所得到的抗性转化株进行了pcr 、 pcr - southernblot 、 southernblot检测，其中2株呈阳性，证明vp4基因已整合到苎麻基因组dna中。
• The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing . but difference was found at 3 bases of the sequence from the reported in genbank . then , an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404 . transgenic tomato were screened by their ability of growing on media containing kanamycin
  本实验采用pcr方法从番茄花cdna文库中克隆到叶绿体shsp基因，经测序证实与genbank中已发表的序列在编码区相差2个碱基，其中一个碱基导致1个氨基酸的改变。将叶绿体shsp基因定向克隆于带有组成性表达启动子camv35s的植物表达载体prok中，冻融法转化农杆菌lba4404 ，利用叶圆盘法对番茄进行ti质粒介导的遗传转化。
• Establishment of carrot genetic transformation an efficient transformation protocol based on agrobacterium tumefacien lba4404 was created in the study . good results were gained when use fresh hypocotyls as infectious explants , co - cultivate in medium supplemented with low concentration of acetosyringone ( 25 m ) , screen in medium with loomg / l kanamycin . it is time saving when anti - culli are fisrt screened in low concentration of antibiotics then transfered to high concentration ones , and remove antibiotics when regenerate
  建立了高效的遗传转化体系以pbi121和ptok233为转化质粒，在农杆菌lba4404介导下，对胡萝卜遗传转化体系进行系统研究，首次为该胡萝卜品种建立一套高效的遗传转化体系，结果为:最适转化受体为新鲜下胚轴及经预培养的下胚轴;共培养时，低浓度的乙酞丁香酮( 25pm )对转化具有促进作用;适宜的卡那霉素筛选浓度是loom岁l 。
• The resulting plasmid , named prok - sod2 , was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation . the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter . transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ) , several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination
  本实验将sod2基因构建到植物表达载体prok中，导入农杆菌后，进行植物遗传转化，实现其在拟南芥中过量表达，在含30mg l的卡那霉素的培养基上筛选获得纯合转基因株系，自交一代获得足够的纯和转基因种子后，对其进行了分子生物学的验证及生理指标的检验。
• The results indicated that all isolates exhibited a susceptibility to amikacin and ceftriaxon , and 67 isolates showed a greater or lesser degree resistance to streptomycin , kanamycin , gentamicin , ampicillin , tetracycline , chloramphenicol , florfenical , cefotaxime , cephalothin and ceftiofur , to which 22 isolates exhibited a susceptibility . some isolates showed resistance to multiple antibiotics and displayed a highest level resistance to streptomycin with a frequency of 43 . 8 % , followed by tetracycline with a frequency of 30 . 3 %
  结果表明，所有分离菌株均对阿米卡星和头孢曲松敏感； 67株对链霉素、卡那霉素、庆大霉素、氨苄西林、四环素、氯霉素、氟苯尼考、头孢噻肟、头孢噻吩、头孢噻呋表现出不同程度的耐药性，其中对链霉素的耐药率最高，为43 . 8 ，其次为四环素( 30 . 3 ) ，其余22株为敏感菌株。
• Our experiment indicates : ( 1 ) the optimal concentration of kanamycin for screening torenia fournier regenerated buds was 400 mg / l . the ideal transformation was obtained in the following conditions : the leaf discs were dipped in agrobacterium suspension that od600 was 0 . 1 for 10 ~ 20 min ; subsequently cocultivated on the ms solid coculture medium containing 20umol / l acetosyringon for 7 to 8 d at 23 , and the induction ratio of regenerated buds was 27 . 2 %
  研究结果表明： ( 1 )筛选蓝猪耳转化芽的最适卡那霉素浓度为400mg l 。 od _ ( 600 )为0 . 1的菌液浓度菌液浸染叶盘10 20min ，固体共培养基(含20 mol l乙酰丁香酮)上23共培养7 8d可获得理想的转化效率，转化芽诱导率为27 . 2 ； 16h光照， 8h黑暗是较理想的共培养光周期；茎段是较好的转化受体。
• The chimeric gene was introduced into arabidopsis through the method of vacuum infiltration which is widely used in arobidopsis tansformation . transgenic plants were screened by means of kanamycin resistance and further identified by genomic pcr and western blotting , through the phenotype observation of transgenic plants , the speculation that apoplast calmodulin may play a certain role in regulating the development and growth of plants was made
  最后，融合基因被插入二元载体plbj21 ，通过电击法转入农杆菌gv3101 ，真空渗入法转入了拟南芥，转基因拟南芥通过kan抗性筛选而得到，并进一步进行了基因组pcr鉴定和western杂交的鉴定。